![]() In addition, the administration of chemotherapeutic agents and multi-drug resistance are accompanied by severe side effects that cause negative gynecological and obstetric outcomes 2, 3. Although the cervical cancer screening program has reduced the incidence and mortality of cervical cancer, the incidence among young women remains a public health problem 1. glyptostroboides floral cones, especially the DME extract, which possesses several anticancer components, as determined by GC–MS analysis, could a potential natural anticancer agent.Ĭervical cancer affects many women in developing countries and has a high mortality rate. Our results suggest that the extracts of M. Furthermore, RT-qPCR and western blotting were used to quantitate the relative RNA and protein levels of the cell death pathway components, respectively. The number of cells at the sub-G1 phase, which indicates apoptotic cells, was increased approximately fourfold upon treatment with the DME and CE extracts compared with that in the negative control. Further, the sub-G1 population was determined by FACS analysis. The viability of COS-7 cells was found to be 12-fold higher than that of the HeLa cells under the administration of 50 µg/ml of the DME extract. The anticancer effect was evaluated by using the Cell Counting Kit-8 assay. HeLa cervical and COS-7 cells were used as a cancer cell model and normal cell control, respectively. glyptostroboides floral cones was subjected to methanol extraction, and the resulting extract was further partitioned by liquid–liquid extraction using the organic solvents n-hexane, dichloromethane (DME), chloroform, and ethyl acetate in addition to deionized water. This study was undertaken to investigate the anticancer effects of organic extracts derived from the floral cones of Metasequoia glyptostroboides.
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